|
KMID : 0545120120220050622
|
|
Journal of Microbiology and Biotechnology
2012 Volume.22 No. 5 p.622 ~ p.627
|
|
|
6-Methoxyluteolin from Chrysanthemum zawadskii var. latilobum Suppresses Histamine Release and Calcium Influx via Down-Regulation of FcεRI α Chain Expression
|
|
Shim Sun-Yup
Park Jeong-Ro
Byun Dae-Seok
|
|
|
Abstract
|
|
|
|
Mast cells and basophils are important effector cells in immunoglobulin-E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-methoxyluteolin, isolated from Chrysanthemum zawadskii, in the FcεRI-mediated allergic reaction. We determined that 6-methoxyluteolin inhibited anti-FcεRI α chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration [Ca2+]i in a dose-dependent manner. Moreover, the inhibitory effects of 6-methoxyluteolin on the cell surface expression and the mRNA level of the FcεRI α chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RTPCR), respectively. Therefore, these results show that 6- methoxyluteolin is a potent inhibitor of histamine release and calcium influx via down-regulation of the FcεRI α chain.
|
|
|
KEYWORD
|
|
|
Chrysanthemum zawadskii var. latilobum, 6-methoxyluteolin, histamine, calcium influx, FcεRI
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|